DETAILS
A COMPLETE GUIDE NAD +
How NAD+ Works
NAD+ operates as essential coenzyme in fundamental cellular processes affecting energy, repair, and longevity.
What is NAD+:
Basic biochemistry:
- Nicotinamide Adenine Dinucleotide (oxidized form)
- Coenzyme present in all living cells
- Exists in two forms: NAD+ (oxidized) and NADH (reduced)
- Acts as electron carrier in metabolic reactions
- Absolutely essential for life
Critical functions:
- Energy metabolism (glycolysis, TCA cycle, electron transport chain)
- DNA repair (PARP enzymes require NAD+)
- Cellular signaling (calcium homeostasis, gene expression)
- Sirtuin activation (longevity proteins)
- Immune function and inflammation regulation
NAD+ is one of the most important molecules in your body.
The Age-Related NAD+ Decline:
The problem:
- NAD+ levels peak in youth
- Decline begins in 20s-30s
- By age 50: ~50% reduction from peak
- By age 80: ~80% reduction
- Progressive depletion with aging
Why NAD+ declines:
- Increased consumption (DNA damage repair requires NAD+)
- Reduced biosynthesis (enzymes decline)
- CD38 enzyme increases (degrades NAD+)
- Chronic inflammation consumes NAD+
- Mitochondrial dysfunction reduces production
Consequences of low NAD+:
- Reduced energy production (fatigue, weakness)
- Impaired DNA repair (cellular damage accumulation)
- Decreased sirtuin activity (accelerated aging)
- Mitochondrial dysfunction (metabolic issues)
- Cognitive decline (brain highly NAD+ dependent)
- Metabolic dysfunction (insulin resistance, obesity)
This decline is considered a central mechanism of aging.
Mitochondrial Energy Production:
NAD+'s role in ATP generation:
The electron transport chain:
- NADH donates electrons to Complex I
- Electron flow through mitochondrial complexes
- Proton gradient created
- ATP synthase produces ATP
- NAD+ regenerated for continued cycling
Without adequate NAD+:
- Electron transport impaired
- ATP production decreases
- Cellular energy declines
- Fatigue and dysfunction result
NAD+ is the electron carrier making energy production possible.
Sirtuin Activation - The Longevity Connection:
What are sirtuins:
- Family of 7 proteins (SIRT1-7)
- NAD+-dependent enzymes (require NAD+ to function)
- Regulate longevity, metabolism, inflammation, stress resistance
- Often called "longevity genes"
- Activated by caloric restriction and exercise
Sirtuin functions:
- SIRT1: Metabolic regulation, inflammation, longevity
- SIRT3: Mitochondrial health, antioxidant defense
- SIRT6: DNA repair, inflammation, longevity
- Others: Various metabolic and protective roles
NAD+ requirement:
- Sirtuins consume NAD+ to function
- Low NAD+ = inactive sirtuins
- Raising NAD+ = sirtuin activation
- This is why NAD+ boosting = longevity intervention
Sirtuins mediate many benefits of caloric restriction and exercise.
DNA Repair Mechanisms:
PARP enzymes (Poly ADP-Ribose Polymerases):
- Detect and repair DNA damage
- Consume NAD+ for energy
- Critical for genomic stability
- Become more active with age (more damage)
The NAD+ drain:
- DNA damage increases with age
- PARPs work harder to repair
- Consume large amounts of NAD+
- Depletes NAD+ available for other functions
- Creates competition between repair and energy/sirtuins
NAD+ restoration:
- Provides fuel for DNA repair
- Maintains genomic integrity
- Reduces cellular senescence
- Supports healthy aging
Metabolic Benefits:
NAD+ in glucose metabolism:
- Required for glycolysis (glucose breakdown)
- Essential for TCA cycle (energy production)
- Regulates insulin sensitivity
- Improves metabolic flexibility
Effects of NAD+ restoration:
- Enhanced glucose metabolism
- Improved insulin sensitivity
- Better mitochondrial function
- Reduced metabolic syndrome markers
Neuroprotection:
Brain's high NAD+ demand:
- Brain highly metabolically active
- Large energy requirements
- Extensive DNA repair needs
- Vulnerable to NAD+ decline
NAD+ neuroprotective effects:
- Enhanced neuronal energy production
- Improved mitochondrial function
- Better DNA repair in neurons
- Sirtuin-mediated neuroprotection
- Reduced neuroinflammation
- May protect against cognitive decline
Direct NAD+ vs. Precursors (NMN, NR):
NAD+ biosynthesis pathway:
- Precursors (NR, NMN) → Converted to NAD+
- Multiple enzymatic steps required
- Conversion efficiency varies
- Some loss in the process
Direct NAD+ administration:
- Provides NAD+ immediately
- Bypasses biosynthesis bottlenecks
- Rapid cellular availability
- No conversion required
Bioavailability considerations:
- Direct NAD+ may be degraded before cellular uptake
- Precursors (NMN/NR) may enter cells better
- Debate ongoing in research
- Both approaches have merit
- Individual response varies
Primary Uses & Applications
1. Anti-Aging & Cellular Longevity (Primary Application)
For age-related decline:
Mechanisms:
- Restores declining NAD+ levels
- Activates sirtuins (longevity pathways)
- Enhances mitochondrial function
- Improves DNA repair capacity
- Reduces cellular senescence
Who benefits:
- Women 40+ experiencing aging effects
- Anyone focused on healthspan extension
- Those interested in cellular anti-aging
- Preventative longevity optimization
- Biohackers and longevity enthusiasts
Realistic expectations:
- Subtle improvements in energy, vitality
- Better cellular health markers over time
- Potential cognitive preservation
- NOT dramatic age reversal
- Long-term preventative strategy
2. Energy Enhancement & Fatigue Reduction
For chronic low energy:
How NAD+ helps:
- Fundamental for mitochondrial ATP production
- Restores cellular energy generation
- Improves metabolic efficiency
- Enhances overall vitality
Applications:
- Chronic fatigue (especially age-related)
- Post-viral fatigue syndromes
- Mitochondrial dysfunction
- General energy optimization
- Mental and physical stamina
Timeline:
- Some notice energy improvements within days to weeks
- Peak benefits with consistent use over months
- Individual variation significant
3. Cognitive Function & Neuroprotection
For brain health:
Effects:
- Enhanced neuronal energy production
- Better mental clarity and focus
- Improved cognitive processing
- Neuroprotective effects
- Potential prevention of cognitive decline
Who uses:
- Brain fog and mental fatigue
- Age-related cognitive slowing
- Those wanting cognitive preservation
- Demanding cognitive work
- Preventative neuroprotection
4. Metabolic Health & Insulin Sensitivity
For metabolic dysfunction:
NAD+ metabolic benefits:
- Improved glucose metabolism
- Enhanced insulin sensitivity
- Better mitochondrial function
- Metabolic flexibility
Applications:
- Insulin resistance
- Metabolic syndrome
- Type 2 diabetes prevention
- Weight management support
- Metabolic optimization
Similar benefits to MOTS-c but different mechanism.
5. Exercise Recovery & Performance
For athletic optimization:
Effects:
- Enhanced mitochondrial function
- Better energy production during exercise
- Improved recovery between sessions
- Reduced exercise-induced oxidative stress
- Greater training adaptations
Who benefits:
- Athletes seeking performance edge
- Masters athletes (40+) maintaining function
- Anyone wanting better recovery
- Intense training periods
6. Addiction Recovery & Neurological Support (Clinical)
IV NAD+ used for:
- Alcohol and substance addiction recovery
- Withdrawal symptom reduction
- Neurological restoration
- Brain healing post-addiction
Injectable NAD+:
- May provide some similar benefits
- Less intensive than IV (lower doses)
- Supportive adjunct therapy
- Medical supervision essential for addiction
Research Dosing Protocols
Direct NAD+ Dosing:
Important: NAD+ dosing differs significantly from precursors (NMN typically 250-500mg daily; NAD+ much lower due to molecular weight and administration route).
Standard Dose Range:
- 50-100mg per injection
- 2-3 times weekly
- Subcutaneous or intramuscular
- Some protocols use higher (200mg+)
Conservative Start:
- 50mg per injection
- 2x weekly
- Assess tolerance and response
- Increase if needed
Higher Dose Protocols:
- 100-200mg per injection
- 2-3x weekly or daily
- More intensive intervention
- For significant fatigue or dysfunction
Frequency Options:
2-3x Weekly (Common):
- Monday/Wednesday/Friday
- OR Tuesday/Thursday/Saturday
- Maintains elevated NAD+ levels
- Convenient, sustainable schedule
Daily (Intensive):
- 50-100mg daily
- Maximum benefit
- For acute issues (severe fatigue, recovery)
- More expensive
- Used in some clinical protocols
Once Weekly (Minimal):
- 100-200mg once weekly
- Maintenance approach
- Some benefit but less optimal
- Budget-friendly option
Timing & Administration:
Flexible timing:
- Morning, afternoon, or evening all work
- No fasting requirement
- Consistency matters
- Some prefer morning (energy for day)
Subcutaneous (Most Common):
- Abdomen, thigh
- Standard insulin syringe
- Easy self-administration
- Good absorption
Intramuscular (Alternative):
- Deeper injection (deltoid, glute, thigh)
- May enhance absorption
- Some users prefer IM
- Either route effective
Cycling:
NAD+ can be used continuously:
- No strict cycling needed
- Long-term safety appears good
- Some prefer cycles for assessment/budget
- Common: 8-12 weeks on, 4 weeks off
- OR continuous indefinitely
Reconstitution:
Standard approach:
Mix with bacteriostatic water or sterile saline.
Example: 5mg vial (note: this is LOW dose - typical NAD+ vials are 50-500mg):
- Mix 5mg with 1ml
- For 50mg dose: Need 10 vials (not practical)
- 5mg vials not typical for NAD+ - usually sold as 50mg, 100mg, 200mg, or 500mg vials
Typical NAD+ reconstitution (100mg vial):
- 100mg in 2ml = 50mg/ml
- For 50mg dose: 1ml
- For 100mg dose: 2ml (full vial)
Vials Needed:
At 50mg 3x weekly (assuming 100mg vials):
- 150mg weekly
- 1.5 vials weekly
- Monthly: ~6-7 vials (100mg each)
At 100mg 3x weekly:
- 300mg weekly
- Monthly: ~12-13 vials (100mg each)
NAD+ moderately to significantly expensive depending on dosing.
Note: The 5mg quantity in product specifications is likely error - NAD+ typically sold in 50-500mg vials due to dosing requirements. Verify actual vial size before purchasing.
Stacking & Combinations
NAD+ + NMN or NR (Comprehensive):
- Injectable NAD+ for immediate boost
- Oral precursors for daily baseline
- Multiple pathway approach
- Maximize NAD+ levels
- Popular among biohackers
NAD+ + Resveratrol or Quercetin:
- Sirtuin activators
- NAD+ fuels sirtuins
- Resveratrol/quercetin activate them
- Synergistic longevity effects
- Classic anti-aging stack
NAD+ + MOTS-c:
- Both support mitochondrial function
- Different mechanisms
- NAD+: Coenzyme for energy production
- MOTS-c: Metabolic regulator
- Complementary mitochondrial support
NAD+ + Metformin:
- Both activate AMPK
- Both improve metabolic health
- Synergistic benefits
- Popular longevity combination
- Medical supervision if diabetic
NAD+ + CoQ10:
- CoQ10: Electron carrier in mitochondria
- NAD+: Electron carrier and more
- Both support energy production
- Mitochondrial optimization
- Complementary mechanisms
NAD+ + Exercise:
- Exercise depletes NAD+ (good stress)
- Supplementation supports recovery
- Enhances training adaptations
- Synergistic health benefits
- Both essential for optimal results
Who This Is/Isn't For
NAD+ is appropriate if you:
- Experiencing age-related energy decline (40+)
- Have chronic fatigue or mitochondrial dysfunction
- Focused on longevity and cellular anti-aging
- Want cognitive enhancement and neuroprotection
- Have metabolic issues or insulin resistance
- Seeking science-based anti-aging intervention
- Can commit to regular injections (2-3x weekly)
- Understand subtle, cellular-level benefits
- Have tried lifestyle optimization (this enhances, doesn't replace)
- Willing to invest in cellular health
NAD+ particularly suited for:
- Women 50+ with significant energy decline
- Chronic fatigue sufferers seeking cellular solution
- Biohackers optimizing longevity protocols
- Those wanting clinical NAD+-like benefits without IV cost
- Anyone prioritizing healthspan over appearance
NOT recommended for:
- Anyone expecting dramatic instant energy (some respond quickly, others don't)
- Those unwilling to commit to injections
- Expecting visible anti-aging (works at cellular level)
- Pregnant, breastfeeding, trying to conceive
- Anyone on very tight budget (moderately expensive)
- Those wanting convenience (oral NMN/NR better choice)
- Anyone expecting replacement for sleep, diet, exercise
Consider oral NMN/NR instead if:
- Prefer daily oral over injections
- Want established research backing
- Seeking budget-friendly long-term option
- New to NAD+ optimization
- Value convenience highly
PRODUCT CERTIFICATES
FREQUENTLY ASKED QUESTIONS
What is NAD+ and why is it important?
NAD+ (Nicotinamide Adenine Dinucleotide) is essential coenzyme in every cell required for: energy production (mitochondrial ATP), DNA repair (PARP enzymes), longevity pathways (sirtuins), metabolic function. Levels decline ~50% by age 50, contributing to aging, fatigue, metabolic dysfunction, cognitive decline. Restoring NAD+ addresses cellular aging at fundamental level.
How is injectable NAD+ different from oral NMN or NR supplements?
Direct NAD+ injection provides active coenzyme immediately, bypassing biosynthesis pathway. NMN/NR are precursors requiring conversion to NAD+ through enzymatic steps. Injectable: Immediate availability, requires injections, less research. Oral precursors: Convenient daily pill, established pathways, more studied. Both approaches valid - debate ongoing about optimal method. Can also combine both.
Will NAD+ give me more energy immediately?
Variable response. Some notice energy improvements within days; others take weeks. NAD+ restores cellular energy production capacity - not stimulant providing forced energy. Works by optimizing mitochondrial function. Expectations: gradual, sustainable energy enhancement over weeks to months. NOT instant dramatic boost for everyone. Individual variation significant.
How does NAD+ help with anti-aging?
Multiple longevity mechanisms: Activates sirtuins (longevity proteins requiring NAD+), enhances DNA repair (prevents damage accumulation), improves mitochondrial function (central to aging), supports metabolic health, reduces cellular senescence. NAD+ decline is hallmark of aging; restoration theoretically slows biological aging. Long-term human data limited but mechanisms well-established.
What's the difference between NAD+ and NADH?
NAD+ is oxidized form; NADH is reduced form. NAD+ accepts electrons (becomes NADH); NADH donates electrons (becomes NAD+). Constantly cycling between forms during metabolism. NAD+/NADH ratio important for cellular function. NAD+ is the form that activates sirtuins and what declines with age. Supplementing NAD+ (not NADH) is goal.
Can NAD+ help with chronic fatigue?
Potentially yes - fatigue often involves mitochondrial dysfunction. NAD+ essential for cellular energy production; restoring levels may improve ATP generation. Used clinically (IV NAD+) for chronic fatigue and post-viral syndromes. Injectable NAD+ may provide similar benefits. Individual response varies - some dramatic improvement, others modest. Address other fatigue causes simultaneously (sleep, thyroid, etc.).
How does NAD+ compare to IV NAD+ therapy?
IV NAD+ delivers very high doses (250-1000mg) directly to bloodstream, immediate high blood levels, dramatic effects reported, expensive ($400-1500 per session). Injectable NAD+: Lower doses (50-200mg), subcutaneous/IM administration, more accessible cost, regular self-administration. IV more intensive; injectable more sustainable long-term. Both restore NAD+ but different intensity.
Will NAD+ improve my cognitive function and brain fog?
May help - brain highly NAD+-dependent (high energy needs, extensive DNA repair). NAD+ restoration supports: neuronal energy production, mitochondrial function, neuroprotection, mental clarity. Many report improved cognition, reduced brain fog, better focus. Effects gradual (weeks). Not dramatic nootropic but cellular support for optimal brain function.
Can I use NAD+ for metabolic health and insulin resistance?
Yes - good application. NAD+ improves: glucose metabolism, insulin sensitivity, mitochondrial function, metabolic flexibility. Research shows metabolic benefits. Similar to MOTS-c but different mechanism (coenzyme vs. peptide regulator). Can combine both for comprehensive metabolic support. Should accompany proper diet and exercise.
Does injection site hurt with NAD+?
NAD+ can cause more injection site discomfort than many peptides - burning/stinging during injection. Varies by individual. Strategies: Inject slowly (30-60 seconds), ice before/after, try IM instead of subQ, tolerance improves with regular use. Discomfort brief (minutes) but noticeable. Not intolerable but worth knowing.
How long can I use NAD+ continuously?
Safety profile suggests long-term use possible - NAD+ naturally occurring, replacing declining levels. Clinical IV NAD+ used for extended periods. No known tolerance or dependency. Common approaches: Continuous indefinitely (2-3x weekly), cycles (8-12 weeks on/4 weeks off), or periodic intensive courses. Long-term human data still developing but appears safe.
Should I take NAD+ or stick with oral NMN/NR?
Depends on preferences and goals. NAD+ injection if: Want immediate NAD+ availability, prefer injectable approach, seeking clinical-like effects, have tried precursors without response. Oral NMN/NR if: Prefer convenience (daily pill), want established research, seeking budget option, new to NAD+ optimization. Both valid - can also combine for comprehensive approach.
Can NAD+ help with addiction recovery like IV NAD+ therapy?
IV NAD+ used clinically for addiction (alcohol, opioids) - helps withdrawal symptoms, neurological restoration, brain healing. Injectable NAD+ provides similar mechanism at lower doses. May help but not replacement for comprehensive addiction treatment. Medical supervision essential for addiction. Injectable supportive adjunct, not standalone solution.
Can I use NAD+ while trying to conceive, pregnant, or breastfeeding?
Not recommended. While NAD+ naturally occurring, high-dose supplementation not studied in pregnancy/breastfeeding. Effects on fetal development unknown. Discontinue if trying to conceive, pregnant, or nursing. Safety not established in these populations.
Important Legal & Safety Information
Regulatory Status:
This product is sold for research purposes. NAD+ is not approved by TGA (Australia) or FDA (USA) as medication. While NAD+ is naturally occurring essential coenzyme, supplemental injectable forms are research products. By purchasing, you acknowledge this product is intended for research use.
Clinical Context:
IV NAD+ therapy used clinically in various settings (addiction treatment, chronic fatigue, anti-aging clinics) with generally good safety profile. Injectable NAD+ provides similar approach at lower doses, more accessible administration. Extensive research on NAD+ biology but limited data specifically on injectable supplementation outside clinical IV settings.
Medical Disclaimer:
While NAD+ is naturally occurring molecule with good safety profile, consultation with healthcare provider recommended if you have: Significant medical conditions, metabolic disorders, are taking medications, chronic fatigue requiring diagnosis, neurological conditions.
Important Distinctions:
NAD+ Supplementation is:
- Cellular energy support
- Anti-aging intervention
- Metabolic optimization
- Longevity strategy
NAD+ is NOT:
- Cure for diseases
- Replacement for medical treatment
- Guaranteed energy solution
- Clinically proven anti-aging therapy (yet)
Realistic Expectations:
NAD+ works at cellular level - effects may be subtle, gradual, accumulating over time. Some notice dramatic energy improvements quickly; others modest benefits over months. Individual variation enormous. Works best as part of comprehensive health optimization including diet, exercise, sleep, stress management. NAD+ enhances fundamentals, doesn't replace them.
Note on Vial Size:
Standard NAD+ dosing (50-200mg) requires larger vials than 5mg. Verify actual vial size before purchase - typical NAD+ products sold as 50mg, 100mg, 200mg, or 500mg vials. If purchasing 5mg vials, confirm this is appropriate concentration for intended use.
Women who are pregnant, breastfeeding, or planning pregnancy should NOT use NAD+.
Must be 18+ to purchase. Users assume all responsibility for handling and use.
Appropriate Use:
NAD+ used for cellular energy optimization, anti-aging, metabolic health,
SHIPPING AND RETURNS
Shipping: Australia Post Express. Orders fulfilled within 24 hours of payment confirmation. Delivery: 2-5 business days Australia-wide. Tracking provided.
Returns: Due to the temperature-sensitive nature of research peptides, we cannot accept returns once shipped. We offer replacements for damaged, incorrect, or lost parcels. Please contact us within 48 hours of delivery for any issues.